验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse liver tissue, mouse skin tissue, mouse thymus tissue, A549 cells, HUVEC cells, rat skin tissue |
| Positive IHC detected in | human cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue |
| Positive IF/ICC detected in | HUVEC cells, MDCK cells, HaCaT cells |
Freshly prepared samples are recommended.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13050-1-AP targets Claudin 1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat, pig, canine, chicken, bovine, sheep, goat |
| 免疫原 |
CatNo: Ag3713 Product name: Recombinant human Claudin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC012471 Sequence: MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 1 |
| 别名 | Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC |
| 计算分子量 | 211 aa, 23 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC012471 |
| 基因名称 | Claudin 1 |
| Gene ID (NCBI) | 9076 |
| RRID | AB_2079881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95832 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
| IHC protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
| WB protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Nat Commun Bioactive nanomotor enabling efficient intestinal barrier penetration for colorectal cancer therapy | ||
J Adv Res Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense. | ||
Neuron Macrophage-derived CTSS drives the age-dependent disruption of the blood-CSF barrier | ||
Gut Microbes Infant feces-derived Lactobacillus gasseri FWJL-4 mitigates experimental necrotizing enterocolitis via acetate production | ||
Gut Microbes Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier. |
