验证数据展示
经过测试的应用
| Positive WB detected in | MDCK cells, mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
26812-1-AP targets CLCN5 in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, Canine, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag25182 Product name: Recombinant human CLCN5 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 627-685 aa of BC130429 Sequence: ESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPLPPYTPPTLKLRNILDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chloride channel 5 |
| 别名 | chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN |
| 计算分子量 | 83 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC130429 |
| 基因名称 | CLCN5 |
| Gene ID (NCBI) | 1184 |
| RRID | AB_2880643 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51795 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CLCN5 antibody 26812-1-AP | Download protocol |
| WB protocol for CLCN5 antibody 26812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
