验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse brain tissue, mouse colon tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy® CLCN3 Ready-To-Use IHC Kit. CLCN3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55330-1-AP targets CLCN3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chloride channel 3 |
| 别名 | ClC 3, CLC3 |
| 计算分子量 | 91 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | NM_001829 |
| 基因名称 | CLCN3 |
| Gene ID (NCBI) | 1182 |
| RRID | AB_3670249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chloride channel 3 (CLCN3), one of the members of the chloride channel family that are voltage-gated, primarily regulates the homeostasis state of intercellular ions and the acidification of cellular compartments. CLCN3 has been shown to function in cell proliferation, migration, and resistance to pharmacological treatment in glioma, ovarian cancer, and stomach adenocarcinoma cells. Therefore, CLCN3 may function critically in the initiation and development of cancer. (PMID: 36439880)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLCN3 antibody 55330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
