验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20803-1-AP targets CLASP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CLASP1 fusion protein Ag14724 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cytoplasmic linker associated protein 1 |
别名 | CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1 |
计算分子量 | 1538 aa, 169 kDa |
观测分子量 | 162 kDa |
GenBank蛋白编号 | BC112940 |
基因名称 | CLASP1 |
Gene ID (NCBI) | 23332 |
RRID | AB_10697808 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q7Z460 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.
实验方案
Product Specific Protocols | |
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WB protocol for CLASP1 antibody 20803-1-AP | Download protocol |
IHC protocol for CLASP1 antibody 20803-1-AP | Download protocol |
IP protocol for CLASP1 antibody 20803-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |