验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HT-29 cells, HepG2 cells, Jurkat cells, SMMC-7721 cells, Y79 cells |
This antibody can recognize both CKS1B and CKS2 simultaneously.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10610-1-AP targets CKS1B/2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0928 Product name: Recombinant human CKS1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-79 aa of BC007751 Sequence: MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CDC28 protein kinase regulatory subunit 1B |
| 别名 | CKS1B, CKS 1, CKS1, CKS-1, ckshs1 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC007751 |
| 基因名称 | CKS1B |
| Gene ID (NCBI) | 1163 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61024 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin-dependent kinase regulatory subunit 1B (CKS1B), a member of the conserved cyclin kinase subunit 1 (CKS1) protein family, plays an essential role in cell cycling. A large number of studies have shown that CKS1B is associated with the pathogenesis of many human cancers and closely related to drug resistance. (PMID: 33102238) The protein CKS1B and CKS2 are very similar. This antibody can recognize both CKS1B and CKS2 simultaneously.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CKS1B/2 antibody 10610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
