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验证数据展示

  • WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

    WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    IF Staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of mouse testis using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of rat brain using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of rat testis using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    IHC staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 60177-1-Ig (same clone as 60177-1-PBS)

    IF Staining of C2C12 using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

CKM-Specific Monoclonal antibody, PBS Only

CKM-Specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60177-1-PBS

产品说明书

CloneNo. 2G3F6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60177-1-PBS targets CKM-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 creatine kinase, muscle
别名 CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM
计算分子量 43 kDa
观测分子量 43 kDa, 90 kDa, 130 kDa
GenBank蛋白编号BC007462
基因名称 CKM
Gene ID (NCBI) 1158
RRIDAB_10897180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06732
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).