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验证数据展示

  • WB analysis of HEK-293 using 18713-1-AP

    WB analysis of HEK-293 using 18713-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18713-1-AP

    IHC staining of mouse brain using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse brain using 18713-1-AP

    IHC staining of mouse brain using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse skeletal muscle using 18713-1-AP

    IHC staining of mouse skeletal muscle using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse testis using 18713-1-AP

    IHC staining of mouse testis using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse skeletal muscle using 18713-1-AP

    IHC staining of mouse skeletal muscle using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included
  • IF Staining of HEK-293 using 18713-1-AP

    IF Staining of HEK-293 using 18713-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18713-1-AP (CKB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human normal colon using 18713-1-AP

    IHC staining of human normal colon using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CKB antibody included

CKB-Specific Polyclonal antibody

CKB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CKB, KCRB, Creatine kinase B-type, CPK-B, CKBB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue, Y79 cells
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human gliomas tissue, mouse skeletal muscle tissue, mouse testis tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18713-1-AP targets CKB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 creatine kinase, brain
别名 CKB, KCRB, Creatine kinase B-type, CPK-B, CKBB
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC001190
基因名称 CKB
Gene ID (NCBI) 1152
RRIDAB_10596470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody is specific to CKB.

实验方案

Product Specific Protocols
WB protocol for CKB-Specific antibody 18713-1-APDownload protocol
IHC protocol for CKB-Specific antibody 18713-1-APDownload protocol
IF protocol for CKB-Specific antibody 18713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols