验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, NIH/3T3 cells, HeLa cells, U-251 cells, C6 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00054 targets CKAP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | cytoskeleton-associated protein 4 |
| 别名 | 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63 |
| 计算分子量 | 602 aa, 66 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC082972 |
| 基因名称 | CKAP4 |
| Gene ID (NCBI) | 10970 |
| RRID | AB_3750106 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | Q07065 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CKAP4 antibody RMX00054 | Download protocol |
| IHC protocol for CKAP4 antibody RMX00054 | Download protocol |
| WB protocol for CKAP4 antibody RMX00054 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
