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验证数据展示

  • WB analysis using 86247-1-RR (same clone as 86247-1-PBS)

    WB analysis using 86247-1-RR (same clone as 86247-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86247-1-RR (CKAP2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 86247-1-RR (same clone as 86247-1-PBS)

    IP experiment of Jurkat using 86247-1-RR (same clone as 86247-1-PBS)

    IP result of anti-CKAP2L (IP:86247-1-RR, 4ug; Detection:86247-1-RR 1:1000) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86247-1-RR (same clone as 86247-1-PBS)

    IF Staining of HepG2 using 86247-1-RR (same clone as 86247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (86247-1-RR, Clone: 250860G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

CKAP2L Recombinant monoclonal antibody, PBS Only

CKAP2L Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 86247-1-PBS

产品说明书

CloneNo. 250860G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CKAP2L Ready-To-Use IHC Kit. CKAP2L primary antibody included.

产品信息

86247-1-PBS targets CKAP2L in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10909

Product name: Recombinant human CKAP2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 396-745 aa of BC036217

Sequence: IRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEEEEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLASKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGFQTPES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytoskeleton associated protein 2-like
别名 Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis
计算分子量 745 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC036217
基因名称 CKAP2L
Gene ID (NCBI) 150468
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYA6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).

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