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验证数据展示

  • WB analysis of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

    WB analysis of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

    IF Staining of SH-SY5Y using 15021-1-AP (same clone as 15021-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

    IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

    IHC staining of mouse brain using 15021-1-AP (same clone as 15021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 15021-1-AP (same clone as 15021-1-PBS)

    IHC staining of mouse ovary using 15021-1-AP (same clone as 15021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15021-1-AP (CITED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15021-1-PBS in a different storage buffer formulation.

CITED2 Polyclonal antibody, PBS Only

CITED2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15021-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15021-1-PBS targets CITED2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7078

Product name: Recombinant human CITED2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC004377

Sequence: MADHMMAMNHGRFPDGTNGLHHHPAHRMGMGQFPSPHHHQQQQPQHAFNALMGEHIHYGAGNMNATSGIRHAMGPGTVNGGHPPSALAPAARFNNSQFMGPPVASQGGSLPASMQLQKLNNQYFNHHPYPHNHYMPDLHPAAGHQMNGTNQHFRDCNPKHSGGSSTPGGSGGSSTPGGSGSSSGGGAGSSNSGGGSGSGNMPASVAHVPAAMLPPNVIDTDFIDEEVLMSLVIEMGLDRIKELPELWLGQNEFDFMTDFVCKQQPSRVSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
别名 MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1
计算分子量 28 kDa
观测分子量 36 kDa, 27 kDa
GenBank蛋白编号BC004377
基因名称 CITED2
Gene ID (NCBI) 10370
RRIDAB_2080545
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99967
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].

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