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验证数据展示

  • WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

    WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

    IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

    WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

CIT Polyclonal antibody, PBS Only

CIT Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20033-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20033-1-PBS targets CIT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 citron (rho-interacting, serine/threonine kinase 21)
别名
计算分子量 231 kDa
观测分子量 230 kDa, 177 kDa
GenBank蛋白编号NM_007174
基因名称 CIT
Gene ID (NCBI) 11113
RRIDAB_10665814
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14578
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

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