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验证数据展示

  • WB analysis using 11057-1-AP

    WB analysis using 11057-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11057-1-AP (Cirhin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 11057-1-AP

    IP experiment of HepG2 using 11057-1-AP

    IP result of anti-Cirhin (IP:11057-1-AP, 4ug; Detection:11057-1-AP 1:1000) with HepG2 cells lysate 1760 ug.

Cirhin Polyclonal antibody

Cirhin Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11057-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

CIRH1A, cPERP-E, KIAA1988, U3 small nucleolar RNA-associated protein 4 homolog, UTP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11057-1-AP targets Cirhin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1494

Product name: Recombinant human CIRH1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 335-686 aa of BC009348

Sequence: RCLISCSKKRQLLLFQFAHHLELWRLGSTVATGKNGDTLPLSKNADHLLHLKTKGPENIICSCISPCGSWIAYSTVSRFFLYRLNYEHDNISLKRVSKMPAFLRSALQILFSEDSTKLFVASNQGALHIVQLSGGSFKHLHAFQPQSGTVEAMCLLAVSPDGNWLAASGTSAGVHVYNVKQLKLHCTVPAYNFPVTAMAIAPNTNNLVIAHSDQQVFEYSIPDKQYTDWSRTVQKQGFHHLWLQRDTPITHISFHPKRPMHILLHDAYMFCIIDKSLPLPNDKTLLYNPFPPTNESDVIRRRTAHAFKISKIYKPLLFMDLLDERTLVAVERPLDDIIAQLPPPIKKKKFGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cirrhosis, autosomal recessive 1A (cirhin)
别名 CIRH1A, cPERP-E, KIAA1988, U3 small nucleolar RNA-associated protein 4 homolog, UTP4
计算分子量 77 kDa
观测分子量 70-77 kDa
GenBank蛋白编号BC009348
基因名称 Cirhin
Gene ID (NCBI) 84916
RRIDAB_2877743
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969X6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN.

实验方案

Product Specific Protocols
WB protocol for Cirhin antibody 11057-1-APDownload protocol
IHC protocol for Cirhin antibody 11057-1-APDownload protocol
IP protocol for Cirhin antibody 11057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols