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验证数据展示

  • WB analysis using 15798-1-AP

    WB analysis using 15798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 15798-1-AP

    IF Staining of MCF-7 using 15798-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 15798-1-AP

    IF Staining of MCF-7 using 15798-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 15798-1-AP

    IHC staining of human stomach cancer using 15798-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15798-1-AP (CIP29 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CIP29 Polyclonal antibody

CIP29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15798-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells, rat pancreas tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15798-1-AP targets CIP29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8507

Product name: Recombinant human CIP29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC007099

Sequence: MATETVELHKLKLAELKQECLARGLETKGIKQDLIHRLQAYLEEHAEEEANEEDVLGDETEEEETKPIELPVKEEEPPEKTVDVAAEKKVVKITSEIPQTERMQKRAERFNVPVSLESKKAARAARFGISSVPTKGLSSDNKPMVNLDKLKERAQRFGLNVSSISRKSEDDEKLKKRKERFGIVTSSAGTGTTEDTEAKKRKRAERFGIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytokine induced protein 29 kDa
别名 Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa
计算分子量 210 aa, 24 kDa
观测分子量 29-32 kDa
GenBank蛋白编号BC007099
基因名称 CIP29
Gene ID (NCBI) 84324
RRIDAB_1959356
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP82979
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CIP29 antibody 15798-1-APDownload protocol
IHC protocol for CIP29 antibody 15798-1-APDownload protocol
IF protocol for CIP29 antibody 15798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Tumour Biol

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Authors - Ying Zhu
View Article
humanWB,IF

PLoS Pathog

TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus

Authors - Lingcai Zhao
  • KD Validated
View Article