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验证数据展示

  • WB analysis using 12132-1-AP (same clone as 12132-1-PBS)

    WB analysis using 12132-1-AP (same clone as 12132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

    IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

    IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 12132-1-AP (same clone as 12132-1-PBS)

    IHC staining of human ovary cancer using 12132-1-AP (same clone as 12132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CIN85 Polyclonal antibody, PBS Only

CIN85 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12132-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12132-1-PBS targets CIN85 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2777

Product name: Recombinant human CIN85 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC015806

Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3-domain kinase binding protein 1
别名 SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3
计算分子量 665 aa, 73 kDa
观测分子量 85 kDa
GenBank蛋白编号BC015806
基因名称 SH3KBP1/CIN85
Gene ID (NCBI) 30011
RRIDAB_2187277
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B97
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

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