CIITA Polyclonal antibody
CIITA Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
55099-1-AP targets CIITA in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | class II, major histocompatibility complex, transactivator |
| Synonyms | C2TA, CIITA, CIITAIV, MHC class II transactivator, MHC2TA, NLRA |
| Calculated Molecular Weight | 124 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_000246 |
| Gene Symbol | CIITA |
| Gene ID (NCBI) | 4261 |
| RRID | AB_2881268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33076 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CIITA, also naned as MHC2TA, is a 1130 amino acid protein, which Interacts with ZXDA and ZXDC. MHC class II molecules are heterodimeric cell surface glycoproteins expressed on B cells, macrophages and dendritic cells, which present antigens to CD4+ T cells. CIITA (class II transactivator) acts as a coactivator for MHC class II-specific gene expression and negatively regulates the IL-4 gene promoter during T cell differentiation. IFNg induces CIITA gene expression via JAK1 and Stat1 pathways. T Defects of CIITA and RFX-B have been implicated as causes of Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIITA antibody 55099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol Identification and validation of m6A-GPI signatures as a novel prognostic model for colorectal cancer |