验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
55099-1-AP targets CIITA in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | class II, major histocompatibility complex, transactivator |
| 别名 | C2TA, CIITA, CIITAIV, MHC class II transactivator, MHC2TA, NLRA |
| 计算分子量 | 124 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | NM_000246 |
| 基因名称 | CIITA |
| Gene ID (NCBI) | 4261 |
| RRID | AB_2881268 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P33076 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CIITA, also naned as MHC2TA, is a 1130 amino acid protein, which Interacts with ZXDA and ZXDC. MHC class II molecules are heterodimeric cell surface glycoproteins expressed on B cells, macrophages and dendritic cells, which present antigens to CD4+ T cells. CIITA (class II transactivator) acts as a coactivator for MHC class II-specific gene expression and negatively regulates the IL-4 gene promoter during T cell differentiation. IFNg induces CIITA gene expression via JAK1 and Stat1 pathways. T Defects of CIITA and RFX-B have been implicated as causes of Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIITA antibody 55099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |