验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32585-1-AP targets CIB2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag38508 Product name: Recombinant human CIB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of NM_006383.3 Sequence: MGNKQTIFTEEQLDNYQDCTFFNKKDILKLHSRFYELAPNLVPMDYRKSPIVHVPMSLIIQMPELRENPFKERIVAAFSEDGEGNLTFNDFVDMFSVLCESAPRELKANYAFKIYDFNTDNFICKEDLELTLARLTKSELDEEEVVLVCDKVIEEADLDGDGKLGFADFEDMIAKAPDFLSTFHIRI* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium and integrin binding family member 2 |
| 别名 | Calcium and integrin-binding family member 2, Kinase-interacting protein 2, KIP 2, KIP2 |
| 计算分子量 | 22 kDa，187aa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | NM_006383.3 |
| 基因名称 | CIB2 |
| Gene ID (NCBI) | 10518 |
| RRID | AB_3742663 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75838 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CIB (calcium and integrin binding) protein family has 4 members (CIB1-CIB4), each composed of 4 EF-hand Ca2+-binding motifs (PMID: 27771768). CIB2 is broadly expressed in a variety of tissues, which plays a role in sensory neurons of the ear and eye involved in the regulation of Ca2+ homeostasis (PMID: 28663585; 29084757; 29255404). Mutations of CIB2 have been associated with autosomal recessive nonsyndromic deafness 48 (DFNB48), as well as syndromic deafness in Usher syndrome, type 1J (USH1J) (PMID: 23331261). The observed molecular weight of CIB2 is 39 kDa because it can form a dimer (PMID: 30174586).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIB2 antibody 32585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
