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验证数据展示

  • WB analysis of HepG2 using 10295-1-AP

    WB analysis of HepG2 using 10295-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 10295-1-AP

    WB analysis of PC-3 using 10295-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 10295-1-AP

    IHC staining of human testis using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 10295-1-AP

    IHC staining of human small intestine using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human small intestine using 10295-1-AP

    IHC staining of human small intestine using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 10295-1-AP

    IF Staining of HepG2 using 10295-1-AP

    Immunofluorescent analysis of HepG2 cells using 10295-1-AP (CIAO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CIAO1 Polyclonal antibody

CIAO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10295-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

WDR39, WD repeat-containing protein 39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10295-1-AP targets CIAO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CIAO1 fusion protein Ag0349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae)
别名 WDR39, WD repeat-containing protein 39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1
计算分子量 38 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC001395
基因名称 CIAO1
Gene ID (NCBI) 9391
RRIDAB_2260509
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76071
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CIAO1, also named as CIA1 or WDR39, is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. It may also play a role in chromosome segregation. The predicted MW of this protein is 38 kDa. 10295-1-AP detected 48-55 kDa band because of phosphorylation. And 10295-1-AP do well in IHC and IF detection.

实验方案

Product Specific Protocols
WB protocol for CIAO1 antibody 10295-1-APDownload protocol
IHC protocol for CIAO1 antibody 10295-1-APDownload protocol
IF protocol for CIAO1 antibody 10295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols