验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Calu-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24444-1-AP targets CHSY3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag19641 Product name: Recombinant human CHSY3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 571-769 aa of BC137325 Sequence: FFRETEELDVNSLVESINSETQSFSFISNSLKILSSFQGAKEMGGHNEKKVHILVPLIGRYDIFLRFMENFENMCLIPKQNVKLVIILFSRDSGQDSSKHIELIKGYQNKYPKAEMTLIPMKGEFSRGLGLEMASAQFDNDTLLLFCDVDLIFREDFLQRCRDNTIQGQQVYYPIIFSQYDPKVTNGGNPPTDDYFIFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chondroitin sulfate synthase 3 |
| 别名 | Carbohydrate synthase 2, chondroitin sulfate synthase 3, Chondroitin synthase 2, ChSy 2, CHSY2 |
| 计算分子量 | 882 aa, 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC137325 |
| 基因名称 | CHSY3 |
| Gene ID (NCBI) | 337876 |
| RRID | AB_3742114 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q70JA7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHSY3 is a Golgi-localized chondroitin sulfate synthase that controls glycosaminoglycan chain elongation on extracellular matrix proteoglycans. By regulating chondroitin sulfate structure, CHSY3 influences matrix organization, growth factor signaling, and tissue mechanics. Its dysregulation is implicated in cartilage pathology, pulmonary remodeling, and cancer progression, highlighting CHSY3 as a key enzymatic regulator of extracellular matrix biology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHSY3 antibody 24444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
