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验证数据展示

  • WB analysis of HepG2 using 13135-1-AP

    WB analysis of HepG2 using 13135-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13135-1-AP (CHST9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13135-1-AP (CHST9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHST9 Polyclonal antibody

CHST9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13135-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13135-1-AP targets CHST9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3874

Product name: Recombinant human CHST9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-438 aa of BC025764

Sequence: NKFSNMNWPVDIHPLNKSLVKDNKWKKTEETQEKRRSFLQEFCKKYGGVSHHQSHLFHTVSRIYVEDKHKILYCEVPKAGCSNWKRILMVLNGLASSAYNISHNAVHYGKHLKKLDSFDLKGIYTRLNTYTKAVFVRDPMERLVSAFRDKFEHPNSYYHPVFGKAIIKKYRPNACEEALINGSGVKFKEFIHYLLDSHRPVGMDIHWEKVSKLCYPCLINYDFVGKFETLEEDANYFLQMIGAPKELKFPNFKDRHSSDERTNAQVVRQYLKDLTRTERQLIYDFYYLDYLMFNYTTPFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9
别名 UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2
计算分子量 438 aa, 52 kDa
观测分子量 52 kDa, 42 kDa
GenBank蛋白编号BC025764
基因名称 CHST9
Gene ID (NCBI) 83539
RRIDAB_2079222
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L1S5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CHST9 antibody 13135-1-APDownload protocol
WB protocol for CHST9 antibody 13135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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