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验证数据展示

  • WB analysis of mouse heart using 16426-1-AP

    WB analysis of mouse heart using 16426-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16426-1-AP (CHST8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 16426-1-AP

    WB analysis using 16426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16426-1-AP (CHST8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skin using 16426-1-AP

    IF Staining of mouse skin using 16426-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse skin using 16426-1-AP

    IF Staining of mouse skin using 16426-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CHST8 Polyclonal antibody

CHST8 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 16426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

EC:2.8.2.-, GalNAc 4 O sulfotransferase 1, GalNAc 4 ST1, GalNAc-4-O-sulfotransferase 1, GalNAc4ST

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, mouse heart tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IF-P detected inmouse skin tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16426-1-AP targets CHST8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9669

Product name: Recombinant human CHST8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 75-424 aa of BC014250

Sequence: VTRDLSSGAPRGRNLPAPDQPQPPLQRGTRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQERKRVMQEACAKYRASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASSTADIQHNTVHYGSALKRLDTFDRQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPNSYYHPVFGKAILARYRANASREALRTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRLCSPCLIDYDFVGKFESMEDDANFFLSLIRAPRNLTFPRFKDRHSQEARTTARIAHQYFAQLSALQRQRTYDFYYMDYLMFNYSKPFADLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
别名 EC:2.8.2.-, GalNAc 4 O sulfotransferase 1, GalNAc 4 ST1, GalNAc-4-O-sulfotransferase 1, GalNAc4ST
计算分子量 424 aa, 49 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC014250
基因名称 CHST8
Gene ID (NCBI) 64377
RRIDAB_2229376
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2A9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHST8 (Carbohydrate sulfotransferase 8), also named GalNAc-4-O-sulfotransferase 1 (GalNAc4-ST1), is a Golgi-resident type II transmembrane enzyme that transfers sulfate to the C-4 hydroxyl group of terminal β1,4-linked N-acetylgalactosamine (GalNAc) residues present in both N- and O-glycans.

实验方案

Product Specific Protocols
IF protocol for CHST8 antibody 16426-1-APDownload protocol
WB protocol for CHST8 antibody 16426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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