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验证数据展示

  • WB analysis of HEK-293 using 17749-1-AP

    WB analysis of HEK-293 using 17749-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17749-1-AP

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 17749-1-AP

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

CHST14 Polyclonal antibody

CHST14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17749-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17749-1-AP targets CHST14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag12014

Product name: Recombinant human CHST14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 63-376 aa of BC049214

Sequence: GILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADLQVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACSNWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVREPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMNEHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
别名 CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST
计算分子量 376 aa, 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC049214
基因名称 CHST14
Gene ID (NCBI) 113189
RRIDAB_2079051
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHST14 antibody 17749-1-APDownload protocol
IHC protocol for CHST14 antibody 17749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Oncol

A comprehensive analysis of the expression, immune infiltration, prognosis and partial experimental validation of CHST family genes in gastric cancer

Authors - Jinbo Zhan
View Article