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验证数据展示

  • WB analysis of BxPC-3 using 12013-1-AP

    WB analysis of BxPC-3 using 12013-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12013-1-AP (CHST10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12013-1-AP

    IHC staining of human gliomas using 12013-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12013-1-AP (CHST10 antibody) at dilution of 1:50 (under 10x lens).

CHST10 Polyclonal antibody

CHST10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12013-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CHST10, HNK 1 sulfotransferase, HNK 1ST, HNK1ST, HuHNK 1ST

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12013-1-AP targets CHST10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2627

Product name: Recombinant human CHST10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-356 aa of BC010441

Sequence: MHHQWLLLAACFWVIFMFMVASKFITLTFKDPDVYSAKQEFLFLTTMPEVRKLPEEKHIPEELKPTGKELPDSQLVQPLVYMERLELIRNVCRDDALKNLSHTPVSKFVLDRIFVCDKHKILFCQTPKVGNTQWKKVLIVLNGAFSSIEEIPENVVHDHEKNGLPRLSSFSDAEIQKRLKTYFKFFIVRDPFERLISAFKDKFVHNPRFEPWYRHEIAPGIIRKYRRNRTETRGIQFEDFVRYLGDPNHRWLDLQFGDHIIHWVTYVELCAPCEIMYSVIGHHETLEDDAPYILKEAGIDHLVSYPTIPPGITVYNRTKVEHYFLGISKRDIRRLYARFEGDFKLFGYQKPDFLLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbohydrate sulfotransferase 10
别名 CHST10, HNK 1 sulfotransferase, HNK 1ST, HNK1ST, HuHNK 1ST
计算分子量 356 aa, 42 kDa
观测分子量 57 kDa
GenBank蛋白编号BC010441
基因名称 CHST10
Gene ID (NCBI) 9486
RRIDAB_2229573
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43529
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHST10 antibody 12013-1-APDownload protocol
IHC protocol for CHST10 antibody 12013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols