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验证数据展示

  • WB analysis using 82848-4-RR

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82848-4-RR

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 82848-4-RR

    FC experiment of SH-SY5Y using 82848-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Jurkat using 82848-4-RR

    FC experiment of Jurkat using 82848-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

CHRNA7 Recombinant monoclonal antibody

CHRNA7 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82848-4-RR

产品说明书

CloneNo. 240117D12
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

240117D12, CHRNA7 2, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82848-4-RR targets CHRNA7 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15980

Product name: Recombinant human CHRNA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 130-321 aa of BC037571

Sequence: TVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cholinergic receptor, nicotinic, alpha 7
别名 240117D12, CHRNA7 2, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7
计算分子量 502 aa, 56 kDa
观测分子量50 kDa, 56 kDa
GenBank蛋白编号BC037571
基因名称 CHRNA7
Gene ID (NCBI) 1139
RRIDAB_3670575
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP36544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

实验方案

Product Specific Protocols
FC protocol for CHRNA7 antibody 82848-4-RRDownload protocol
WB protocol for CHRNA7 antibody 82848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Neuromolecular Med

Hypertension Induces Cognitive Impairment and Alterations of Acetylcholine Receptor mRNA Expression in the Hippocampus and Cortex of Mice

Authors - Jingfeng Liu
View Article
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