验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21939-1-AP targets CHRNA4 in WB, ELISA applications and shows reactivity with human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, Mouse |
| 免疫原 |
CatNo: Ag16584 Product name: Recombinant human CHRNA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-573 aa of BC096290 Sequence: SPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cholinergic receptor, nicotinic, alpha 4 |
| 别名 | BFNC, CHRNA4, EBN, EBN1, NACHR, NACHRA4, NACRA4 |
| 计算分子量 | 627 aa, 70 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC096290 |
| 基因名称 | CHRNA4 |
| Gene ID (NCBI) | 1137 |
| RRID | AB_3085680 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43681 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHRNA4 antibody 21939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
