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验证数据展示

  • WB analysis of Jurkat using 21586-1-AP

    WB analysis of Jurkat using 21586-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 21586-1-AP

    WB analysis of Jurkat using 21586-1-AP

    WB result of CHRNA7/CHRFAM7A antibody (21586-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7/CHRFAM7A transfected Jurkat cells.

  • Featured Product
  • KD/KO验证

CHRNA7/CHRFAM7A Polyclonal antibody

CHRNA7/CHRFAM7A Polyclonal Antibody for WB, ELISA
Cat No. 21586-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21586-1-AP targets CHRNA7/CHRFAM7A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16151

Product name: Recombinant human CHRFAM7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 241-386 aa of BC101348

Sequence: TRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
别名 CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10
计算分子量 412 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC101348
基因名称 CHRNA7/CHRFAM7A
Gene ID (NCBI) 89832
RRIDAB_10734326
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ494W8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.

实验方案

Product Specific Protocols
WB protocol for CHRNA7/CHRFAM7A antibody 21586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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