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  • Featured Product
  • KD/KO Validated

CHPF N-terminal Polyclonal antibody

CHPF N-terminal Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23953-1-AP

Print datasheet

Synonyms

Chondroitin sulfate synthase 2, CHPF, CHPF N-terminal, CHSY2, CSS2

验证数据展示

  • WB analysis of human placenta using 23953-1-AP

    WB analysis of human placenta using 23953-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 23953-1-AP

    IF Staining of HepG2 using 23953-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 23953-1-AP

    IHC staining of human colon cancer using 23953-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 23953-1-AP

    IHC staining of human colon cancer using 23953-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse skeletal muscle tissue, C6 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23953-1-AP targets CHPF N-terminal in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CHPF N-terminal fusion protein Ag20819 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chondroitin polymerizing factor
Synonyms Chondroitin sulfate synthase 2, CHPF, CHPF N-terminal, CHSY2, CSS2
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85-90 kDa, 65-70 kDa
GenBank Accession NumberBC023531
Gene Symbol CHPF
Gene ID (NCBI) 79586
RRIDAB_2879373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IZ52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
IHC protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
IF protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

Targeting Chondroitin Sulfate Reduces Invasiveness of Glioma Cells by Suppressing CD44 and Integrin β1 Expression.

Authors - Yin-Hung Chu
View Article
humanIHC,WB

J Cell Commun Signal

Chondroitin polymerizing factor predicts a poor prognosis and promotes breast cancer progression via the upstream TGF-β1/SMAD3 and JNK axis activation

Authors - Qiang-Feng Pan
View Article
humanWB,IHC,IF

Aging (Albany NY)

The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma

Authors - Haitao Luo
  • KD Validated
View Article