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验证数据展示

  • ChIP experiment of NIH/3T3 using 81462-1-RR (same clone as 81462-1-PBS)

    ChIP experiment of NIH/3T3 using 81462-1-RR (same clone as 81462-1-PBS)

    Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/ml, overnigh). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of CHOP (81462-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

    WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

    Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81462-1-RR

    Affinity and Kinetic Characterization of 81462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

    {{ptg:ProductFeaturesInfo}}

CHOP/GADD153 Recombinant monoclonal antibody, PBS Only

CHOP/GADD153 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA, ChIP-qPCR
Cat No. 81462-1-PBS

产品说明书

CloneNo. 1G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA, ChIP-qPCR

别名

CHOP; GADD153, CHOP, DDIT3, GADD153, 1G11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.

产品信息

81462-1-PBS targets CHOP/GADD153 in WB, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7354

Product name: Recombinant human CHOP; GADD153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-169 aa of BC003637

Sequence: MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA-damage-inducible transcript 3
别名 CHOP; GADD153, CHOP, DDIT3, GADD153, 1G11
计算分子量 19 kDa
观测分子量30 kDa
GenBank蛋白编号BC003637
基因名称 CHOP
Gene ID (NCBI) 1649
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35638
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).