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验证数据展示

  • WB analysis using 68811-2-Ig (same clone as 68811-2-PBS)

    WB analysis using 68811-2-Ig (same clone as 68811-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68811-2-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68811-2-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 68811-2-Ig (same clone as 68811-2-PBS)

    IF Staining of HCT 116 using 68811-2-Ig (same clone as 68811-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-2-Ig, Clone: 2A3F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68811-2-PBS in a different storage buffer formulation.

CHMP4B Monoclonal antibody, PBS Only

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68811-2-PBS

产品说明书

CloneNo. 2A3F5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHMP4B Ready-To-Use IHC Kit. CHMP4B primary antibody included.

产品信息

68811-2-PBS targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag33487

Product name: Recombinant human CHMP4B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC033859

Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromatin modifying protein 4B
别名 C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2
计算分子量 224 aa, 25 kDa
GenBank蛋白编号BC033859
基因名称 CHMP4B
Gene ID (NCBI) 128866
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H444
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

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