验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84881-2-RR targets Choline Kinase Alpha in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4761 Product name: Recombinant human CHKA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-439 aa of BC036471 Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | choline kinase alpha |
| 别名 | CHKA, EC:2.7.1.32, CKI, CHK, CHETK-alpha |
| 计算分子量 | 439 aa, 52 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC036471 |
| 基因名称 | CHKA |
| Gene ID (NCBI) | 1119 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Choline Kinase Alpha antibody 84881-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
