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Choline Kinase Alpha Polyclonal antibody

Choline Kinase Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

CHKA, CHETK-alpha, CHK, CKI, EC:2.7.1.32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inCOLO 320 cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Choline Kinase Alpha fusion protein Ag4761 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 choline kinase alpha
别名 CHKA, CHETK-alpha, CHK, CKI, EC:2.7.1.32
计算分子量 439 aa, 52 kDa
观测分子量 50 kDa
GenBank蛋白编号BC036471
基因名称 CHKA
Gene ID (NCBI) 1119
RRIDAB_2079160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IHC protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IF protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IP protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Proc Natl Acad Sci U S A

The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation

Authors - Qingxia Ma
mouseWB, IHC

Ann Rheum Dis

Choline kinase inhibition in rheumatoid arthritis.

Authors - M Guma
humanWB

Clin Cancer Res

Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.

Authors - Roopali Saxena
humanIHC

Cell Mol Life Sci

Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling

Authors - Kunxiang Gong
mouseWB

Front Cell Dev Biol

Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.

Authors - Ming Kong
ratWB

J Biol Chem

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.

Authors - Arsenault Daniel J DJ
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