验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Choline Kinase Alpha fusion protein Ag4761 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | choline kinase alpha |
| 别名 | CHKA, CHETK-alpha, CHK, CKI, EC:2.7.1.32 |
| 计算分子量 | 439 aa, 52 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC036471 |
| 基因名称 | CHKA |
| Gene ID (NCBI) | 1119 |
| RRID | AB_2079160 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| IHC protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| IF protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| IP protocol for Choline Kinase Alpha antibody 13520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation | ||
Clin Cancer Res Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment. | ||
Cell Mol Life Sci Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling | ||
Front Cell Dev Biol Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration. | ||
J Biol Chem ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells. |
