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验证数据展示

  • WB analysis using 80056-2-RR (same clone as 80056-2-PBS)

    WB analysis using 80056-2-RR (same clone as 80056-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80056-2-RR (Chk1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293T using 80056-2-RR (same clone as 80056-2-PBS)

    IF Staining of HEK-293T using 80056-2-RR (same clone as 80056-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using Chk1 antibody (80056-2-RR, Clone: 2G7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

Chk1 Recombinant monoclonal antibody, PBS Only

Chk1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 80056-2-PBS

产品说明书

CloneNo. 2G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

CHEK1, 2G7, Checkpoint kinase-1, CHEK 1, Chk 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

80056-2-PBS targets Chk1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, 2G7, Checkpoint kinase-1, CHEK 1, Chk 1
计算分子量 54 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO14757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

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