验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13402-1-AP targets CHD9 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4245 Product name: Recombinant human CHD9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC033770 Sequence: MTDPMMDFFDDANLFGETLEGLSDDAFVQPGPVSLVDELNLGAEFEPLHIDSLNHVQGTPTHQKMTDFEQLNQFDSIKFHHVNQSFGSPAEHVLSPHSQFNCSPIHPQNQPNGLFPDVSDGSPMWGHQTATTISNQNGSPFHQQGHSHSMHQNKSFVAHHDFALFQANEQQTQCTSLRSQQNRNNLNPGQNSLSQSKNFMNVSGPHRVNVNHPPQMTNASNSQQSISMQQFSQTSNPSAHFHKCSSHQEGNFNGPSPNMTSCSVSNSQQFSSHYSFSSNHISPNSLLQSSAVLASNHTNQTLSDFTGSNSFSPHRGIKQESTQHILNPNTSLNSNNFQILHSSHPQGNYS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain helicase DNA binding protein 9 |
| 别名 | AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator |
| 计算分子量 | 2897 aa, 326 kDa |
| 观测分子量 | 330 kDa |
| GenBank蛋白编号 | BC033770 |
| 基因名称 | CHD9 |
| Gene ID (NCBI) | 80205 |
| RRID | AB_10640425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3L8U1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IHC protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IF protocol for CHD9 antibody 13402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Carcinog miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.
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Braz J Med Biol Res Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer. | ||
Int J Clin Exp Pathol High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma. | ||
mBio A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.
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