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验证数据展示

  • WB analysis using 31919-1-AP

    WB analysis using 31919-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 31919-1-AP (CHD7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 31919-1-AP

    IF Staining of HEK-293 using 31919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CHD7 antibody (31919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CHD7 Polyclonal antibody

CHD7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31919-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA1416, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 7, Chromodomain Helicase DNA Binding Protein 7, CHD-7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HuH-7 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31919-1-AP targets CHD7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CHD7 fusion protein Ag36595 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 7
别名 KIAA1416, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 7, Chromodomain Helicase DNA Binding Protein 7, CHD-7
观测分子量350 kDa
GenBank蛋白编号BC110818
基因名称 CHD7
Gene ID (NCBI) 55636
RRIDAB_3670144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2D1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent eukaryotic chromatin remodeling enzyme that regulates nucleosome positioning and alters DNA accessibility, and is essential for organ development.CHD7 is a gene known to be associated with CHARGE syndrome, Kallmann syndrome, and hypogonadotropic hypogonadism, where it is associated with CHARGE syndrome is a congenital multiorgan disorder characterized by eye defects, heart defects, posterior nasal atresia, growth retardation, genital anomalies, ear malformations, and deafness. The effects of CHD7 mutations on inner ear development, neuronal differentiation, cardiovascular development, and regulation of bone lipid homeostasis have been studied.

实验方案

Product Specific Protocols
WB protocol for CHD7 antibody 31919-1-APDownload protocol
IF protocol for CHD7 antibody 31919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols