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验证数据展示

  • WB analysis using 86077-2-RR (same clone as 86077-2-PBS)

    WB analysis using 86077-2-RR (same clone as 86077-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86077-2-RR (CHD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86077-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86077-2-RR (same clone as 86077-2-PBS)

    IHC staining of mouse brain using 86077-2-RR (same clone as 86077-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86077-2-RR (CHD3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86077-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHD3 antibody included
  • IF Staining of HeLa using 86077-2-RR (same clone as 86077-2-PBS)

    IF Staining of HeLa using 86077-2-RR (same clone as 86077-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD3 antibody (86077-2-RR, Clone: 250644A12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86077-2-PBS in a different storage buffer formulation.

CHD3 Recombinant monoclonal antibody, PBS Only

CHD3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86077-2-PBS

产品说明书

CloneNo. 250644A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ATP-dependent helicase CHD3, CHD 3, CHD-3, Chromodomain-helicase-DNA-binding protein 3, EC:3.6.4.12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHD3 Ready-To-Use IHC Kit. CHD3 primary antibody included.

产品信息

86077-2-PBS targets CHD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 3
别名 ATP-dependent helicase CHD3, CHD 3, CHD-3, Chromodomain-helicase-DNA-binding protein 3, EC:3.6.4.12
计算分子量 227 kDa
观测分子量 250 kDa
GenBank蛋白编号NM_005852
基因名称 CHD3
Gene ID (NCBI) 1107
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12873
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

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