验证数据展示
经过测试的应用
| Positive WB detected in | pig heart tissue, A549 cells, HeLa cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat brain tissue |
| Positive IHC detected in | human lung tissue, human lung cancer tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat heart tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68259-1-Ig targets CHCHD3/MIC19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag22628 Product name: Recombinant human CHCHD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-227 aa of BC014839 Sequence: MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil-helix-coiled-coil-helix domain containing 3 |
| 别名 | CHCHD3, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MIC19, MICOS complex subunit MIC19, MINOS3 |
| 计算分子量 | 227 aa, 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC014839 |
| 基因名称 | CHCHD3 |
| Gene ID (NCBI) | 54927 |
| RRID | AB_2935344 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NX63 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHCHD3/MIC19 antibody 68259-1-Ig | Download protocol |
| IHC protocol for CHCHD3/MIC19 antibody 68259-1-Ig | Download protocol |
| IF protocol for CHCHD3/MIC19 antibody 68259-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
