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验证数据展示

  • WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • Featured Product
  • KD/KO验证

CHCHD2 Monoclonal antibody, PBS Only

CHCHD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66302-1-PBS

产品说明书

CloneNo. 2G1G10

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2G1G10, AAG10, Aging-associated gene 10 protein, C7orf17, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

产品信息

66302-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag24219

Product name: Recombinant human CHCHD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-151 aa of BC003079

Sequence: MPRGSRSRTSRMAPPASRAPQMRAAPRPAPVAQPPAAAPPSAVGSSAAAPRQPGLMAQMATTAAGVAVGSAVGHTLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCRLANGLA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 2
别名 2G1G10, AAG10, Aging-associated gene 10 protein, C7orf17, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2
计算分子量 151 aa, 16 kDa
观测分子量 17 kDa
GenBank蛋白编号BC003079
基因名称 CHCHD2
Gene ID (NCBI) 51142
RRIDAB_2881685
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6H1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).