Co-IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:19424-1-AP 1:600) with HepG2 cells lysate 1400ug.

Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

HAP1 (WT and CHCHD10 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 25671-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

WB result of CHCHD10 antibody (25671-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD10 transfected HEK-293 cells.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HAP1 WT cells (yellow outline) and CHCHD10 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 25671-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

HAP1 lysates prepared and IP of CHCHD10 performed using 1.0 μg of 25671-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:25671-1-AP 1:600) with mouse heart tissue lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).