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KD/KO Validated

CHCHD10 Polyclonal antibody

CHCHD10 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 25671-1-AP

Print datasheet

Synonyms

C22orf16, CHCHD10, N27C7 4, Protein N27C7 4

验证数据展示

WB analysis using 25671-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25671-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 using 25671-1-AP
HAP1 (WT and CHCHD10 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 25671-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human kidney using 25671-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 25671-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 25671-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HAP1 using 25671-1-AP
HAP1 WT cells (yellow outline) and CHCHD10 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 25671-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HAP1 using 25671-1-AP
HAP1 lysates prepared and IP of CHCHD10 performed using 1.0 μg of 25671-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of mouse heart using 25671-1-AP
IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:25671-1-AP 1:600) with mouse heart tissue lysate 4000ug.
IF Staining of HeLa using 25671-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 25671-1-AP

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经过测试的应用

Positive WB detected in	HEK-293 cells, mouse brain tissue, HAP1, mouse heart tissue, rat heart tissue
Positive IP detected in	mouse heart tissue, HAP1 cells
Positive IHC detected in	human kidney tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, HAP1

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 6 publications below

产品信息

25671-1-AP targets CHCHD10 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IF, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, chicken
Immunogen	CHCHD10 fusion protein Ag22598 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	coiled-coil-helix-coiled-coil-helix domain containing 10
Synonyms	C22orf16, CHCHD10, N27C7 4, Protein N27C7 4
Calculated Molecular Weight	14 kDa
Observed Molecular Weight	14 kDa
GenBank Accession Number	BC065232
Gene Symbol	CHCHD10
Gene ID (NCBI)	400916
RRID	AB_2880187
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WYQ3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
WB protocol for CHCHD10 antibody 25671-1-AP	Download protocol
IHC protocol for CHCHD10 antibody 25671-1-AP	Download protocol
IF protocol for CHCHD10 antibody 25671-1-AP	Download protocol
IP protocol for CHCHD10 antibody 25671-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IF	Nat Commun Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Authors - Jung-A A Woo KD Validated View Article
human	WB	Nat Commun TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro. Authors - Minwoo Baek View Article
mouse	IHC	J Mol Cell Biol Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum. Authors - Jian Peng View Article
mouse	WB	Diabetes CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis Authors - Meng Ding KO Validated View Article
mouse	WB	Mol Ther Nucleic Acids Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease. Authors - Colleen S Stein View Article
human	WB,IF	Hum Mol Genet Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS. Authors - Isabella R Straub KD Validated View Article

CHCHD10 Polyclonal antibody

CHCHD10 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis using 25671-1-AP

WB analysis of HEK-293 using 25671-1-AP

WB analysis of HAP1 using 25671-1-AP

IHC staining of human kidney using 25671-1-AP

IHC staining of human kidney using 25671-1-AP

IF Staining of HeLa using 25671-1-AP

IF Staining of HAP1 using 25671-1-AP

IP experiment of HAP1 using 25671-1-AP

IP experiment of mouse heart using 25671-1-AP

IF Staining of HeLa using 25671-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.

TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.

Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.

CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis

Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.

Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.