验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16304-1-AP targets CHAC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag9281 Product name: Recombinant human CHAC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC019239 Sequence: MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPAGCVWGVAYRLPVGKEEEVKAYLDFREKGGYRTTTVIFYPKDPTTKPFSVLLYIGTCDNPDYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELANSIRNLVPEEADEHLFALEKLVKERLEGKQNLNCI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ChaC, cation transport regulator homolog 2 (E. coli) |
| 别名 | Glutathione-specific gamma-glutamylcyclotransferase 2, Gamma-GCG 2, EC:4.3.2.7, Cation transport regulator-like protein 2 |
| 计算分子量 | 184 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC019239 |
| 基因名称 | CHAC2 |
| Gene ID (NCBI) | 494143 |
| RRID | AB_2229405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUX2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHAC2 is a gamma-glutamyl cyclotransferase that catalyzes the conversion of glutathione to 5-oxoproline and cysteinylglycine. CHAC2 is involved in continuous, basal turnover of cytosolic glutathione(PMID: 27913623).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CHAC2 antibody 16304-1-AP | Download protocol |
| WB protocol for CHAC2 antibody 16304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cells Adh Promotes Actinobacillus pleuropneumoniae Survival in Porcine Alveolar Macrophages by Inhibiting CHAC2-Mediated Respiratory Burst and Inflammatory Cytokine Expression | ||
Environ Toxicol Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.
| ||
J Cancer CHAC2 promotes lung adenocarcinoma by regulating ROS-mediated MAPK pathway activation
| ||
Mol Neurobiol NUS1 Variants Cause Lennox-Gastaut Syndrome Related to Unfolded Protein Reaction Activation | ||
J Mol Histol SGLT2 inhibitor downregulated oxidative stress via activating AMPK pathway for cardiorenal (CR) protection in CR syndrome rodent fed with high protein diet | ||
Int J Biol Sci Febuxostat therapy improved the outcomes of cardiorenal syndrome rodent through alleviating xanthine oxidase-induced oxidative stress and mitochondrial dysfunction |
