验证数据展示
经过测试的应用
Positive WB detected in | rat kidney tissue, L02 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
16101-1-AP targets CGRRF1 in WB, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, rat |
文献引用反应性 | human |
免疫原 | CGRRF1 fusion protein Ag8998 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cell growth regulator with ring finger domain 1 |
别名 | CGR19, CGRRF1, RING finger protein 197, RNF197 |
计算分子量 | 332 aa, 38 kDa |
观测分子量 | 42-48 kDa |
GenBank蛋白编号 | BC015063 |
基因名称 | CGRRF1 |
Gene ID (NCBI) | 10668 |
RRID | AB_2878219 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99675 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CGRRF1 is a growth suppressor and consists of a transmembrane domain and a RING-finger domain. It functions as a RING domain E3 ubiquitin ligase involved in endoplasmic reticulum-associated degradation. The expression of CGRRF1 is decreased in cancer tissues.(PMID: 31801577).
实验方案
Product Specific Protocols | |
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WB protocol for CGRRF1 antibody 16101-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |