验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse kidney tissue, mouse small intestine tissue, MCF-7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21369-1-AP targets Cingulin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 |
CatNo: Ag15843 Product name: Recombinant human CGN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC146657 Sequence: MAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cingulin |
| 别名 | KIAA1319, CGN |
| 计算分子量 | 1197 aa, 136 kDa |
| 观测分子量 | 136-150 kDa |
| GenBank蛋白编号 | BC146657 |
| 基因名称 | Cingulin |
| Gene ID (NCBI) | 57530 |
| RRID | AB_10733647 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2M7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cingulin antibody 21369-1-AP | Download protocol |
| IP protocol for Cingulin antibody 21369-1-AP | Download protocol |
| WB protocol for Cingulin antibody 21369-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Nat Commun Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.
| ||
Mucosal Immunol Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity. | ||
Cancers (Basel) miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN. | ||
Sci Rep Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats | ||
Cell Signal Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate. |
