验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse thymus tissue, K-562 cells, Raji cells, HL-60 cells, Jurkat cells, rat thymus tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10716-1-AP targets CGGBP1 in WB, IHC, IF, IP, CoIP, chIP, IP-MS, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, chIP, IP-MS, Dot Blot |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1129 Product name: Recombinant human CGGBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC005222 Sequence: MERFVVTAPPARNRSKTALYVTPLDRVTEFGGELHEDGGKLFCTSCNVVLNHVRKSAISDHLKSKTHTKRKAEFEEQNVRKKQRPLTASLQCNSTAQTEKVSVIQDFVKMCLEANIPLEKADHPAVRAFLSRHVKNGGSIPKSDQLRRTYLPDGYENENQLLNSQDC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CGG triplet repeat binding protein 1 |
| 别名 | 20 kDa CGG-binding protein, CGG triplet repeat-binding protein 1, CGG-binding protein 1, CGGBP, p20-CGGBP DNA-binding protein |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19-23 kDa |
| GenBank蛋白编号 | BC005222 |
| 基因名称 | CGGBP1 |
| Gene ID (NCBI) | 8545 |
| RRID | AB_2079283 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UFW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CGGBP1 antibody 10716-1-AP | Download protocol |
| WB protocol for CGGBP1 antibody 10716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep The CGG triplet repeat binding protein 1 counteracts R-loop induced transcription-replication stress.
| ||
BMC Cancer An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1. | ||
Transcription CGGBP1 from higher amniotes restricts cytosine methylation and drives a GC-bias in transcription factor-binding sites at repressed promoters |
