验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse thymus tissue, K-562 cells, Raji cells, HL-60 cells, Jurkat cells, rat thymus tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10716-1-AP targets CGGBP1 in WB, IHC, IF, IP, chIP, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IP, chIP, Dot Blot |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | CGGBP1 fusion protein Ag1129 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CGG triplet repeat binding protein 1 |
别名 | 20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein |
计算分子量 | 19 kDa |
观测分子量 | 19-23 kDa |
GenBank蛋白编号 | BC005222 |
基因名称 | CGGBP1 |
Gene ID (NCBI) | 8545 |
RRID | AB_2079283 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UFW8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)
实验方案
Product Specific Protocols | |
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WB protocol for CGGBP1 antibody 10716-1-AP | Download protocol |
IHC protocol for CGGBP1 antibody 10716-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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BMC Cancer An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1. | ||
BMC Genet CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity. |