验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20738-1-AP targets CFTR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) |
| 别名 | EC:5.6.1.6, Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7 |
| 计算分子量 | 168 kDa |
| GenBank蛋白编号 | NM_000492 |
| 基因名称 | CFTR |
| Gene ID (NCBI) | 1080 |
| RRID | AB_2722558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13569 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the human cytogenetic location of the CFTR gene?
7q31.2
What is the predicted molecular weight of CFTR?
168 kDa
Where is CFTR expressed?
CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.
What is the ion selectivity of CFTR?
CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.
What is the process of CFTR trafficking?
The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.
What are the separate domains of CFTR?
CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.
What regulates CFTR?
CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.
What is the role of CFTR?
CFTR functions as a channel for anions, regulating water and ionic homeostasis.
What mutations in CFTR leads to protein degradation?
Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.
How does CFTR differ from other ATP-binding cassette proteins?
Unlike other proteins in the ABC family, CFTR functions as an ion channel.
PMIDs: 24513531, 24534272, 22966013, 22709980
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CFTR antibody 20738-1-AP | Download protocol |
| IF protocol for CFTR antibody 20738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
PLoS One Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet. | ||
J Exp Clin Cancer Res Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer | ||
Int Immunopharmacol A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma | ||
Biochem Biophys Res Commun Dapagliflozin administration to a mouse model of type 2 diabetes induces DNA methylation and gene expression changes in pancreatic islets | ||
Asian J Androl The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities |
