验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Planning an IHC experiment? We recommend our IHCeasy CFL2 Ready-To-Use IHC Kit. CFL2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2408 Product name: Recombinant human CFL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC022876 Sequence: MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cofilin 2 (muscle) |
| 别名 | CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2 |
| 计算分子量 | 166 aa, 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC022876 |
| 基因名称 | Cofilin 2 |
| Gene ID (NCBI) | 1073 |
| RRID | AB_2080914 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y281 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cofilin 2 antibody 11848-1-AP | Download protocol |
| IHC protocol for Cofilin 2 antibody 11848-1-AP | Download protocol |
| WB protocol for Cofilin 2 antibody 11848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells. | ||
Noncoding RNA Res ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma |
