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验证数据展示

  • WB analysis using 16583-1-AP (same clone as 16583-1-PBS)

    WB analysis using 16583-1-AP (same clone as 16583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

    IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

    IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 16583-1-AP (same clone as 16583-1-PBS)

    IP experiment of HepG2 using 16583-1-AP (same clone as 16583-1-PBS)

    IP result of anti-CFHR3 (IP:16583-1-AP, 4ug; Detection:16583-1-AP 1:300) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

CFHR3 Polyclonal antibody, PBS Only

CFHR3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16583-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16583-1-PBS targets CFHR3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9963

Product name: Recombinant human CFHR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC058009

Sequence: MLLLINVILTLWVSCANGQVKPCDFPDIKHGGLFHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDYIHCTQNGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQPICINSSEKCGPPPPISNGDTTSFLLKVYVPQSRVEYQCQPYYELQGSNYVTCSNGEWSEPPRCIHPCIITEENMNKNNIKLKGRSDRKYYAKTGDTIEFMCKLGYNANTSILSFQAVCREGIVEYPRCE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement factor H-related 3
别名
计算分子量 330 aa, 37 kDa
观测分子量 45-56 kDa
GenBank蛋白编号BC058009
基因名称 CFHR3
Gene ID (NCBI) 10878
RRIDAB_2080151
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02985
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

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