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验证数据展示

  • IP experiment of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    IP experiment of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    IP result of anti-CFAP70 (IP:31344-1-AP, 4ug; Detection:31344-1-AP 1:300) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 31344-1-AP (same clone as 31344-1-PBS)

    IHC staining of rat testis using 31344-1-AP (same clone as 31344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    IHC staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    IF Staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CFAP70 antibody (31344-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

CFAP70 Polyclonal antibody, PBS Only

CFAP70 Polyclonal Antibody for IHC, IF-P, IP, Indirect ELISA
Cat No. 31344-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31344-1-PBS targets CFAP70 in IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35209

Product name: Recombinant human TTC18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC032856

Sequence: MEQVPSAGRLVQITVTEGYDLKGFKGDTPVTFIRAEFNQVVLGDSAKITVSPEGSAKYNFTSSFEFNPEGGITSDDLAHKPVFLTVTEVLPKEKKQKEEKTLILGQAVVDLLPLLEGQSSFQTTVPLHPVQGSPLETPRSSAKQCSLEVKVLVAEPLLTTAQISGGNLLKVTLEAAYSVPESFIPTGPGQNYMVGLQVPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 18
别名
计算分子量126 kDa
观测分子量 126 kDa
GenBank蛋白编号BC032856
基因名称 TTC18
Gene ID (NCBI) 118491
RRIDAB_3669954
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T0N1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).

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