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验证数据展示

  • WB analysis using 30579-1-AP

    WB analysis using 30579-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30579-1-AP (CFAP47 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30579-1-AP

    IHC staining of mouse kidney using 30579-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30579-1-AP

    IHC staining of mouse kidney using 30579-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30579-1-AP

    IHC staining of mouse testis using 30579-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30579-1-AP

    IHC staining of mouse testis using 30579-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 30579-1-AP

    IF Staining of hTERT-RPE1 using 30579-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CFAP47 antibody (30579-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CFAP47 Polyclonal antibody

CFAP47 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30579-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CXorf59, Cilia and flagella associated protein 47

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMRC-5 cells, human testis tissue, mouse spermatophore tissue
Positive IHC detected inmouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30579-1-AP targets CFAP47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31679

Product name: Recombinant human CXorf59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC101700

Sequence: MAIHLDKQNIILKNDKDEYLKKTRDGVLPPYQDAKPPSPASIKKTYTTSKFNDAEPAKGNLFIGVEVLPENLHLDESETSEEDHGSLEKEKYEQFLSLEEGTKAHYFFEKVVNAAQTWFSLFGWPEGPHSFSIPETIRRDVYKMQFYSSTSPPQKFSRQNDFSKYNKTIYDVLLHLSGKMPPGINSSQSLPVDNHEKRVIQLHLQHSSLLDFLNAQGGCISHVLPEFLLEPEDYKRWIEIMSSTNTMPVSSCTPKKKCSIVIEMSKFEAWSKRAWTDVFLQIYKVLVLSRVVPYCSNNMPPICVQNTPKVNPCFASSNIYSDSERILLSWMNINYENTRHVIWKNCHKDVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome X open reading frame 59
别名 CXorf59, Cilia and flagella associated protein 47
观测分子量100-110 kDa
GenBank蛋白编号BC101700
基因名称 CXorf59
Gene ID (NCBI) 286464
RRIDAB_3742352
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZTR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CFAP47 (Cilia- and flagella-associated protein 47), formerly known as CXorf22, CXorf59, is located on the human chromosome X and highly expressed in the testis and encodes a cilia- and flagella-associated protein. CFAP47 was expressed in primary cilia of human kidney tubules, and knockout (KO) mice exhibited vacuolation of tubular cells and tubular dilation, providing evidence that CFAP47 is a causative gene involved in cyst formation (PMID: 33472045, 38633811). CFAP47-mutated male mice are sterile, with decreased sperm motility and abnormal flagella morphology (PMID: 37424856).

实验方案

Product Specific Protocols
IF protocol for CFAP47 antibody 30579-1-APDownload protocol
IHC protocol for CFAP47 antibody 30579-1-APDownload protocol
WB protocol for CFAP47 antibody 30579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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