验证数据展示
经过测试的应用
| Positive WB detected in | MG-63 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32922-1-AP targets CFAP221 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38512 Product name: Recombinant human hCG_17324 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-508 aa of NM_001271049.1 Sequence: LEEFERLNTLSKKVNVPPEKAMMHINFHRPPAKPKPQKVKEIEYQNLRFPVDLSNPFAVATVLNQEPGKLKIKELREVLDQGTEISKTRQMKEALFEQKVRQDIHEEMENHLKWQVHLGKDPMSFKLKKELTEEWQKACAKYKLDRGDPILDEEFQRLKTEVSHKRVVRNQEEKIKEFHPTFDPLINNTWLSRSRAQKRFQQVARKVMIQGRLFNMLSAVREMDKESILRKIGQAKQSIAQEAN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | primary ciliary dyskinesia protein 1 |
| 别名 | Cilia- and flagella-associated protein 221, PCDP1, Primary ciliary dyskinesia protein 1 |
| 计算分子量 | 97kDa，840a |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | NM_001271049.1 |
| 基因名称 | hCG_17324 |
| Gene ID (NCBI) | 200373 |
| RRID | AB_3742788 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q4G0U5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CFAP221 (also known as PCDP1) is a crucial structural protein in motile cilia and sperm flagella. It is specifically located within the axillary shaft of the cilia and acts as a "connection bridge" between the adjacent outer doublet microtubules, playing a role in stabilizing the core skeleton of the axillary shaft and coordinating the sliding of microtubules. The loss of this protein's function will disrupt the structural integrity and normal oscillation of the cilia, leading to a genetic disorder known as primary ciliary dyskinesia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CFAP221 antibody 32922-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
