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验证数据展示

  • WB analysis of HepG2 using 19520-1-AP

    WB analysis of HepG2 using 19520-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19520-1-AP (CES7-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19520-1-AP

    IF Staining of HepG2 using 19520-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19520-1-AP (CES7-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CES7-Specific Polyclonal antibody

CES7-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Carboxylesterase 7, CES7, Carboxylesterase 5A, CES5, CES4C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19520-1-AP targets CES7-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxylesterase 7
别名 Carboxylesterase 7, CES7, Carboxylesterase 5A, CES5, CES4C1
计算分子量 64 kDa
观测分子量 58-60 kDa
GenBank蛋白编号NM_145024
基因名称 CES7
Gene ID (NCBI) 221223
RRIDAB_10642002
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NT32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CES7, also named as CAUXIN, CES4C1, CES5 and FLJ31547, belongs to the type-B carboxylesterase/lipase family. CES7 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES7 catalyze the reaction: A carboxylic ester + H2O = an alcohol + a carboxylate. The antibody can recognize the isoform1 and isoform2.

实验方案

Product Specific Protocols
WB protocol for CES7-Specific antibody 19520-1-APDownload protocol
IF protocol for CES7-Specific antibody 19520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols