验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse liver tissue |
| Positive IHC detected in | mouse heart tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
25731-1-AP targets CERK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CERK fusion protein Ag22774 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ceramide kinase |
| 别名 | Acylsphingosine kinase, ceramide kinase, CERK, dA59H18.2, dA59H18.3, hCERK, KIAA1646, Lipid kinase 4, LK4 |
| 计算分子量 | 537 aa, 60 kDa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC126940 |
| 基因名称 | CERK |
| Gene ID (NCBI) | 64781 |
| RRID | AB_2880214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCT0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CERK antibody 25731-1-AP | Download protocol |
| IHC protocol for CERK antibody 25731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Burns Identification of the potential role of PANoptosis-related genes in burns via bioinformatic analyses and experimental validation | ||
Ecotoxicol Environ Saf The PPARβ/CERK/C1P signaling pathway is a potential mechanism by which antimony exposure promotes prostate cancer cell proliferation |
