验证数据展示
产品信息
68112-1-PBS targets CEP89, CCDC123 as part of a matched antibody pair:
MP51731-1: 68112-1-PBS capture and 68112-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IF-P, Cytometric bead array, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28339 Product name: Recombinant human CCDC123 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 248-343 aa of BC136328 Sequence: MDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 123 |
| 别名 | CCDC123, CEP89, 1F12C5, Centrosomal protein 123, Centrosomal protein of 89 kDa |
| 计算分子量 | 783 aa, 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC136328 |
| 基因名称 | CEP89 |
| Gene ID (NCBI) | 84902 |
| RRID | AB_2923641 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96ST8 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).
